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 | Acceso al texto completo restringido a Biblioteca INIA Treinta y Tres. Por información adicional contacte bibliott@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha : |
12/09/2014 |
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Actualizado : |
08/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
CARLOMAGNO, M.; MATHO, C.; CANTOU, G.; SANBORN, J.R.; LAST, J.A.; HAMMOCK, B.D.; ROEL, A.; GONZÁLEZ, D.; GONZÁLEZ-SAPIENZA, G. |
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Afiliación : |
MARIA GUILLERMINA CANTOU MAYOL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ALVARO ROEL DELLAZOPPA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
A clomazone immunoassay to study the environmental fate of the herbicide in rice (Oryza sativa) agriculture. |
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Fecha de publicación : |
2010 |
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Fuente / Imprenta : |
Journal of Agricultural and Food Chemistry, 2010, v. 58, no.7, p. 4367-4371. |
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ISSN : |
0021-8561 |
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DOI : |
10.1021/jf9043259 |
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Idioma : |
Inglés |
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Notas : |
Article history: received for review December 7, 2009. Revised manuscript received March 8, 2010. Accepted March 10, 2010. Published on Web 03/19/2010. |
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Contenido : |
Abstract:
The environmental impact of rice agriculture is poorly studied in developing countries, mainly due to limitations of the analytical capacity. Here, we report the development of a clomazone enzymelinked immunosorbent assay as a fast and cost-effective tool to monitor the dissipation of this herbicide along the harvest. Antibodies were prepared using different strategies of hapten conjugation, and the best hapten/antibody pair was selected. It proved to be a reliable tool to measure the herbicide in the 2.0-20 ng/mL range in field samples, with excellent correlation with highperformance liquid chromatography results. The assay was used to study the dissipation of the herbicide in the floodwater of experimental rice paddies in Uruguay. Large differences in the residual amounts of herbicide were observed depending on the flooding practices. Because of its robustness and simplicity, the assay may be useful to delineate and monitor management practices that can
contribute to minimizing the release of the herbicide in the environment. |
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Palabras claves : |
ELISA; HPLC; RICE. |
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Thesagro : |
ARROZ; CLOMAZONE; CONTROL DE MALEZAS; ESCARDA; HERBICIDAS; ORYZA SATIVA. |
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Asunto categoría : |
H60 Malezas y escardas |
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Marc : |
LEADER 02181naa a2200361 a 4500
001 1050191
005 2019-10-08
008 2010 bl uuuu u00u1 u #d
022 $a0021-8561
024 7 $a10.1021/jf9043259$2DOI
100 1 $aCARLOMAGNO, M.
245 $aA clomazone immunoassay to study the environmental fate of the herbicide in rice (Oryza sativa) agriculture.$h[electronic resource]
260 $c2010
500 $aArticle history: received for review December 7, 2009. Revised manuscript received March 8, 2010. Accepted March 10, 2010. Published on Web 03/19/2010.
520 $aAbstract: The environmental impact of rice agriculture is poorly studied in developing countries, mainly due to limitations of the analytical capacity. Here, we report the development of a clomazone enzymelinked immunosorbent assay as a fast and cost-effective tool to monitor the dissipation of this herbicide along the harvest. Antibodies were prepared using different strategies of hapten conjugation, and the best hapten/antibody pair was selected. It proved to be a reliable tool to measure the herbicide in the 2.0-20 ng/mL range in field samples, with excellent correlation with highperformance liquid chromatography results. The assay was used to study the dissipation of the herbicide in the floodwater of experimental rice paddies in Uruguay. Large differences in the residual amounts of herbicide were observed depending on the flooding practices. Because of its robustness and simplicity, the assay may be useful to delineate and monitor management practices that can contribute to minimizing the release of the herbicide in the environment.
650 $aARROZ
650 $aCLOMAZONE
650 $aCONTROL DE MALEZAS
650 $aESCARDA
650 $aHERBICIDAS
650 $aORYZA SATIVA
653 $aELISA
653 $aHPLC
653 $aRICE
700 1 $aMATHO, C.
700 1 $aCANTOU, G.
700 1 $aSANBORN, J.R.
700 1 $aLAST, J.A.
700 1 $aHAMMOCK, B.D.
700 1 $aROEL, A.
700 1 $aGONZÁLEZ, D.
700 1 $aGONZÁLEZ-SAPIENZA, G.
773 $tJournal of Agricultural and Food Chemistry, 2010$gv. 58, no.7, p. 4367-4371.
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INIA Treinta y Tres (TT) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela; INIA Las Brujas. |
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Fecha actual : |
22/07/2018 |
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Actualizado : |
09/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
SILVEIRA, C.S.; CESAR, D.; KEATING, M.K.; DELEON-CARNES, M.; ARMIÉN, A.; LUHERS, M.; RIET-CORREA, F.; GIANNITTI, F. |
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Afiliación : |
CAROLINE DA SILVA SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DEBORAH CESAR, Private Practice, Uruguay; M. KELLY KEATING, Centers for Disease Control and Prevention, Atlanta, GA, USA; MARLENE DELEON-CARNES, Centers for Disease Control and Prevention, Atlanta, GA, USA; ANÍBAL ARMIÉN, Universidad de Minnesota; MARTÍN LUHERS, Universidad de la República (UdelaR); FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
A case of prototheca zopfii genotype 1 infection in a dog (Canis lupus familiaris). |
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Complemento del título : |
Case Report. |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
Mycopathologia, June 2018. [First Online] |
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ISSN : |
0301-486X / Online: 1573-0832 |
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DOI : |
10.1007/s11046-018-0274-5 |
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Idioma : |
Inglés |
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Notas : |
Article history: First Online: 06 June 2018. |
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Contenido : |
ABSTRACT.
Protothecosis is a rare disease caused by environmental algae of the genus Prototheca. These are saprophytic, non-photosynthetic, aerobic, colorless algae that belong to the Chlorellaceae family. Seven different species have been described. Prototheca zopfii genotype 2 and P. wickerhamii are most commonly involved in pathogenic infections in humans and animals. The objective of this work is to describe, for the first time, a case of protothecosis caused by P. zopfii genotype 1 in a dog. The dog, a 4-year-old mix bred male, was presented to a veterinary clinic in Montevideo, Uruguay, with multiple skin nodules, one of which was excised by surgical biopsy. The sample was examined histologically and processed by PCR, DNA sequencing, and restriction fragments length polymorphisms for the detection and genotyping of P. zopfii. In addition, transmission electron microscopy and scanning electron microscopy were performed. Histology showed severe ulcerative granulomatous dermatitis and panniculitis with myriads of pleomorphic algae. Algal cells were 4?17 µm in size, with an amphophilic, 2?4-µm-thick wall frequently surrounded by a clear halo, contained flocculant material and a deeply basophilic nucleus, and internal septae with daughter cells (endospores) consistent with endosporulation. Ultrastructurally, algal cells/endospores at different stages of development were found within parasitophorous vacuoles in macrophages. Prototheca zopfii genotype 1 was identified by molecular testing, confirming the etiologic diagnosis of protothecosis. © Springer MenosABSTRACT.
Protothecosis is a rare disease caused by environmental algae of the genus Prototheca. These are saprophytic, non-photosynthetic, aerobic, colorless algae that belong to the Chlorellaceae family. Seven different species have been described. Prototheca zopfii genotype 2 and P. wickerhamii are most commonly involved in pathogenic infections in humans and animals. The objective of this work is to describe, for the first time, a case of protothecosis caused by P. zopfii genotype 1 in a dog. The dog, a 4-year-old mix bred male, was presented to a veterinary clinic in Montevideo, Uruguay, with multiple skin nodules, one of which was excised by surgical biopsy. The sample was examined histologically and processed by PCR, DNA sequencing, and restriction fragments length polymorphisms for the detection and genotyping of P. zopfii. In addition, transmission electron microscopy and scanning electron microscopy were performed. Histology showed severe ulcerative granulomatous dermatitis and panniculitis with myriads of pleomorphic algae. Algal cells were 4?17 µm in size, with an amphophilic, 2?4-µm-thick wall frequently surrounded by a clear halo, contained flocculant material and a deeply basophilic nucleus, and internal septae with daughter cells (endospores) consistent with endosporulation. Ultrastructurally, algal cells/endospores at different stages of development were found within parasitophorous vacuoles in macrophages. Prototheca zopfii genotype 1 was identified by mol... Presentar Todo |
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Palabras claves : |
Algal diseases; Algal infection; Dog; ENFERMEDADES CAUSADAS POR ALGAS; Genotypic characterization; PLATAFORMA SALUD ANIMAL; PROTOTECOSIS; Protothecosis; SOUTH AMERICA. |
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Thesagro : |
ENFERMEDADES INFECCIOSAS; PERRO; SUD AMERICA. |
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Asunto categoría : |
--
L73 Enfermedades de los animales |
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Marc : |
LEADER 02685naa a2200385 a 4500
001 1058833
005 2019-10-09
008 2018 bl uuuu u00u1 u #d
022 $a0301-486X / Online: 1573-0832
024 7 $a10.1007/s11046-018-0274-5$2DOI
100 1 $aSILVEIRA, C.S.
245 $aA case of prototheca zopfii genotype 1 infection in a dog (Canis lupus familiaris).
260 $c2018
500 $aArticle history: First Online: 06 June 2018.
520 $aABSTRACT. Protothecosis is a rare disease caused by environmental algae of the genus Prototheca. These are saprophytic, non-photosynthetic, aerobic, colorless algae that belong to the Chlorellaceae family. Seven different species have been described. Prototheca zopfii genotype 2 and P. wickerhamii are most commonly involved in pathogenic infections in humans and animals. The objective of this work is to describe, for the first time, a case of protothecosis caused by P. zopfii genotype 1 in a dog. The dog, a 4-year-old mix bred male, was presented to a veterinary clinic in Montevideo, Uruguay, with multiple skin nodules, one of which was excised by surgical biopsy. The sample was examined histologically and processed by PCR, DNA sequencing, and restriction fragments length polymorphisms for the detection and genotyping of P. zopfii. In addition, transmission electron microscopy and scanning electron microscopy were performed. Histology showed severe ulcerative granulomatous dermatitis and panniculitis with myriads of pleomorphic algae. Algal cells were 4?17 µm in size, with an amphophilic, 2?4-µm-thick wall frequently surrounded by a clear halo, contained flocculant material and a deeply basophilic nucleus, and internal septae with daughter cells (endospores) consistent with endosporulation. Ultrastructurally, algal cells/endospores at different stages of development were found within parasitophorous vacuoles in macrophages. Prototheca zopfii genotype 1 was identified by molecular testing, confirming the etiologic diagnosis of protothecosis. © Springer
650 $aENFERMEDADES INFECCIOSAS
650 $aPERRO
650 $aSUD AMERICA
653 $aAlgal diseases
653 $aAlgal infection
653 $aDog
653 $aENFERMEDADES CAUSADAS POR ALGAS
653 $aGenotypic characterization
653 $aPLATAFORMA SALUD ANIMAL
653 $aPROTOTECOSIS
653 $aProtothecosis
653 $aSOUTH AMERICA
700 1 $aCESAR, D.
700 1 $aKEATING, M.K.
700 1 $aDELEON-CARNES, M.
700 1 $aARMIÉN, A.
700 1 $aLUHERS, M.
700 1 $aRIET-CORREA, F.
700 1 $aGIANNITTI, F.
773 $tMycopathologia, June 2018. [First Online]
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